Frozen FMT Instructions
Frozen FMT allows you the flexibility that a donor doesn’t. But it is reported not to have the same ‘hit’ as fresh as the quality is compromised by freezing in home refrigerators.
There are three ways to freeze FMT. When you do a fresh FMT you can pour some of the slurry into ice cubes and keep them to use as needed.
Add a few drops of liquid glycerol (or vegetable glycerine) to preserve it, but not too much as it is a laxative. This has been shown to keep the bacterial cell walls from bursting during the freezing process. Without glycerine, you will lose species.
However to maximise the potency of the FMT it is best to freeze the sample without adding water. The more interference (air and water) the more the potency is compromised. To do this, you distribute the sample into ice cubes or cupcake trays. Alternately freeze it whole and break it up later by putting it into two plastic bags and hitting it with a hammer.
The amount of frozen FMT you use will depend on how much you have and when you will next see your donor. There are no hard and fast rules. Considering that pro-biotics come in tiny capsules you really don’t need much FMT to make a difference, especially if you are doing it regularly.
To defrost FMT put it in a cup of warm distilled water/saline and keep stirring until it’s dissolved. If you find this too revolting then put it in the blender. The water should be warm enough so that the end mix will be tepid, a comfortable temperature to have inside you, but not so hot that the good bacteria are killed. If the mix is too cold once defrosted, simply add a little warm water. Once it’s defrosted strain the mix into an enema bag as per above instructions. Where possible, always do FMT when your bowel has been emptied.
We don’t yet know how long frozen stool can be kept. One person has used it after 10 months without adverse affects. OpenBiome states that although further research has yet to be done “microbiological culturing experience suggests that samples may be stored for up to 6 months at -20°C without a significant loss of viability”.
Click Here for How to Make Microbiota Capsules